Medical Laboratory Reagents |
| Binuclease |
| CAS No. 9025-65-4 EC No. 3.1.30.2 |
| from yeast cells with cloned gene encoding genetically engineered Serratia marcescens |
| Catalog/Part No. |
CT-B-021 |
| Synonyms |
Serratia Marcescens Binuclease; Unrestricted Endonuclease |
| Properties |
|
| pH range |
pH 6~10
optimum pH 8.0 |
| Storage Temperature |
-20 °C for lyophilized powder |
| Form |
lyophilized powder |
| Temperature profile |
Optimum temperature 37°C |
| Temperature range of enzyme activity: |
0~42°C |
| Specific activity |
= 1000 kU/mg protein |
| Unit definition: |
According to the protocol from Sigma-Aldrich, one unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ?A260nm of 1.0 in 30 minutes at pH 8.0 at 37°C. |
| Activity |
= 20 kU/mg lyophilized powder
= 250 U/µL buffered aqueous glycerol solution |
| Purity and Quality |
|
| Protease: |
None detected |
| Endotoxin (Tachypleus Amebocyte Lysate): |
None Detected |
| Product Description |
|
| General Description |
Binuclease is a genetically engineered endonuclease from Serratia marcescens gene that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Binuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester. |
| Advantages |
Binuclease is produced from eukaryotic yeast cells that avoid the contamination of endotoxin from prokaryotic cells. The specific activity of Binuclease was meatured at = 1000 kU/mg protein, which is much higher than any existing products (as much as 200%). Binuclease is highly stable under harsh industrial conditions and very cost effective, which make it a perfect alternative for Benzonase. |
| Application: |
Binuclease is an ideal tool to remove nucleic acids (DNA and RNA) in biological products or reduce viscosity in cell lysates. Endonuclease is widely used in the pharmaceutical industry to eliminate nucleic acid contamination in vaccine and protein products. |
| Preparation Notes |
|
| Activators |
1~10 mM Mg2+ |
| Stability |
lyophilized powder is stable for 3 years when stored below 4°C. Buffered aqueous glycerol solution is stable for 1 years when stored at -20°C. |
| Transportation and Storage: |
|
| Buffered aqueous glycerol solution: |
transport with blue ice (below 4°C ). Storage Recommended at -20°C |
| lyophilized powder: |
transport at room temperature. Storage Recommended below 4°C. |
| Storage buffer: |
20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl, 50% glycerol. |
| Dilution buffer: |
20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl. |
| Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
